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White Paper hiPSC mimicking the lumenized rosette epiblast pluripotent stem cells in vivo

Release of the White Paper on the hiPSC lumenized rosette

Induced hPSCs are often touted as an infinite source of cells for research, disease modelling, and therapeutic applications. Being adherent cells, hPSCs have originally been cultured as 2D colonies in Petri dishes or T-flasks. However, despite their apparent unlimited proliferation capacity, hPSCs only exist for a few days in vivo and do not grow as 2D colonies. In vivo, pluripotency (here restricted to human primed pluripotency) is only observed during the second week of human development, and exclusively within 3D epithelialized arrangements of cells.

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